Neurite Outgrowth Inhibitor Protein B (NogoB)

Many tissues such as muscle, skin, liver, and peripheral nerve, have remarkable ability to repair and regrow after injury. The CNS (brain and spinal cord) is limited in its ability to repair or regrow causing permanent brain damage or paralyses. An inhibitory myelin protein, Nogo (Neurite outgrowth), has been cloned and characterized. It may help block the regeneration of the CNS. Nogo is the 4th member of reticulon (Rtn) family. There are three alternative isoforms of Nogo. Nogo-A is full length human protein consisting of 1192aa, Mr ~135kD (rat 1163aa). Nogo-B is an intermediate form consisting of 373aa, ~37kD. Nogo-B lacks aa186-1004 within the extracellular domain. Nogo-C is shorter, consisting of 199aa, ~25kD. It lacks aa186-1004 and has a smaller, alternative N-terminal domain. It is similar to rat vp20 and foocen-s. Nogo-A has a putative extracellular domain of 1024aa, 2-3 TM domains, and a short C-terminus of 43aa. Nogo-A is localized to the CNS-myelin. It is highly expressed in oligodendrocytes, but not by Schwann cells. Nogo-B and Nogo-C have been found in several non-neuronal tissues (skeletal muscle, kidney, skin, lung, and spleen).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nWestern Blot: 1-10ug/ml (ECL). \nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nN2116-41: Neurite Outgrowth Inhibitor Protein B, Rat \n(Nogo-B)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2116-42

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Chen, M.S., et al., Nature 403: 434-439 (2000). 2. GrandPre, T., et al., Nature 403: 439-444 (2000). 3. Goldberg, J.L. and Barres, B.A., Nature 403: 369-370 (2000). 4. Prinjha, R., et al., Nature 403: 383-384 (2000). 5. Tessier-Lavigne, M. and Goodman, C.S., Science 287: 813-814 (2000). 6. Nagase, T., et al., DNA Res. 5: 355-364 (1998).

Additional Info

Recognizes rat Neurite Outgrowth Inhibitor Protein B (MW~37kD). Species Sequence Homology: Mouse: 100%; Human: 83%.