Neuro?lament L,H, 70kD, 200kD (NF-L+H)

The 70kD neuro?lament antigen is present in neurons and tumor cells of neuronal origin. Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200kD) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70kD NF-L protein can self-assemble into a filamentous structure, however the 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. \n\nApplications:\nImmunohistochemistry: (frozen)- 1:40, (paraffin)- 1:40. Does not require antigen retrieval using heat treatment prior to staining of paraffin sections.\nOptimal working dilutions to be determined by researcher.\n\nRecommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP conjugate \n\nRecommended Positive Control Tissue: Cerebellum\n\nRecommended Negative Controls: Mouse IgG1 Negative Control\n

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SPECIFICATIONS

Catalog Number

N2168

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified IgG

Isotype

IgG1

Additional Info

Recognizes human brain neurofilaments of 70kD and 200kD. Stains neurons in the central and peripheral nervous systems. Neuroblastomas and ganglioneuromas are positively labeled.\n