Neurocan (Neurocan Core Protein, Chondroitin Sulfate Proteoglycan 3, Ncan, Cspg3) (Biotin)

Neurocan (Neural proteoglycan; also CSPG3) is a secreted, 300-350kD proteoglycan member of the lectican family of molecules. It is expressed by neurons and astrocytes, and has multiple binding partners, including heparin, FGF2, HB-CAM, glypican-1, syndecan-3, and tenascin-C plus -R. Its expression is highest during development where it regulates neuronal migration and neurite outgrowth. Mature mouse Neurocan is 1246aa in length. It is highly modular, containing a V-type Ig-like domain (aa43-157), two link modules (aa159-356), two EGF-like domains (aa960-1034), a C-type lectin region (aa1036-1165), and a Sushi domain (aa1165-1225). Cleavage occurs between Met643 and Leu644, generating a 621aa, 170-190kD N-terminal fragment, and a 625aa, 180kD C-terminal fragment. Without proteoglycan adducts, SDS-page shows full-length Neurocan to be 245-260kD in size, with the N-terminal fragment 130kD, and the C-terminal fragment 150kD in size.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.1-0.2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2137-01

Size

50ug

Applications

WB

Hosts

Sheep

Reactivities

Mouse, Rat

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes mouse Neurocan, Species Crossreactivity: rat.