NeuroD (Neurogenic Differentiation Factor, Helix-loop-helix Transcription Factor)

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG (1,2). Neuronal activity results in CaMKII-mediated phosphorylation of NeuroD at Ser336, which is necessary for formation and growth of dendrites (3,4). NeuroD is also phosphorylated at Ser274 though the results are context dependent as phosphorylation by ERK stimulates NeuroD activity in pancreatic Beta-cells while phosphorylation by GSK3-Beta inhibits NeuroD in neurons (3). NeuroD is crucially important in both the pancreas and developing nervous system in addition to playing a large role in the development of the inner ear and mammalian retina (3). Mice lacking NeuroD become severely diabetic and die shortly after birth due to Beta-cell differentiation defects (2,3,5,6). The lack of NeuroD in the brain results in severe defects in development (5). Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young (1,3).\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% \n Tween-20 at 4 degrees C with gentle shaking, overnight. \nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2142-95B

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol and (same/less than)0.02% sodium azide.

P Type

Mab

Isotype

IgG

References

(1) Schonhoff, S.E. et al. (2004) Endocrinology 145, \n2639-2644. \n (2) Sharma, A. et al. (1999) Mol. Cell Biol. 19, 704-713. \n (3) Chae, J.H. et al. (2004) Mol. Cells 18, 271-288. \n (4) Gaudillie?re, B. et al. (2004) Neuron 41, 229-241. \n (5) Miyata, T. et al. (1999) Genes Dev. 13, 1647-1652. \n (6) Naya, F.J. et al. (1997) Genes Dev. 11, 2323-2334.

Additional Info

Recognizes human NeuroD. Species crossreactivity: rat. Sequence Homology: mouse.