Neuroendocrine Specific Protein A (NSP-A, Rtn 1A, Reticulon 1A)

Recently, a novel gene family has been identified and characterized, the protein products of which are localized in the endoplasmic reticulum. The proteins encoded by these genes were therefore named Reticulons. Reticulon-1 (Rtn-1) was formerly designated NSP (Neuroendocrine-Specific-Protein), because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. NSP-A, NSP-B, and NSP-C. These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions.NSP-A is a protein with a molecular weight (MW) of about 135kD, which occurs in various isoforms presumably depending on the degree of phosphorylation in serine residues. In lung cancer diagnosis NSP-A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of NSP-A. NSP-B is a phosphoprotein with a MW of 45kD and is restricted to the lung cancer cell line NCI-H82. NSP-B is not found for so far in human tissues. NSP-C is a protein with a MW of 23kD which is not phosphorylated and is found with NSP-A in SCLC (cell lines) and not in non-SCLC (cell cultures).\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50-1:100\nImmunohistochemistry: 1:50-1:100; Frozen and paraffin-embedded tissue sections with avidin-biotinylated horseradish peroxidase complex (ABC) as the detection reagent.\nWestern Blot: 1:50-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2146-01

Size

100ug

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Van de Velde, H.J., et al., Mol. Brain Res. 23: 81-92 (1994). 2. Van de Velde, H.J., et al., J. Cell Sci. 107: 2403-2416 (1994). 3. Van de Velde, H.J., et al., Cancer Res. 54: 4769-4776 (1994). 4. Senden, N.H., et al., J. Pathol. 182: 13-21 (1997). 5. Hens, J., et al., Cell Tissue Res. 292: 229-237 (1998).

Additional Info

Recognizes the 135kD Neuroendocrine Specific Protein A (NSP-A) in immunoblots of NCI-H82 and other SCLC cell lines. Stains normal and pathological neural and neuroendocrine tissues. The epitope is located between aa174-337. \nSpecies Crossreactivity: Human, mouse, rat and hamster.