Neuroendocrine Specific Protein AB (NSP-AB, Rtn 1AB, Reticulon 1AB)

Recently, a novel gene family has been identified and characterized, the protein products of which are localized in the endoplasmic reticulum. The proteins encoded by these genes were therefore named Reticulons. \nReticulon-1 (Rtn-1) was formerly designated NSP (Neuroendocrine-Specific-Protein), because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. NSP-A, NSP-B, and NSP-C. These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions.\nNSP-A is a protein with a molecular weight (MW) of about 135kD, which occurs in various isoforms presumably depending on the degree of phosphorylation in serine residues. In lung cancer diagnosis NSP-A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of NSP-A. NSP-B is a phosphoprotein with a MW of 45kD and is restricted to the lung cancer cell line NCI-H82. NSP-B is not found for so far in human tissues. NSP-C is a protein with a MW of 23kD which is not phosphorylated and is found with NSP-A in SCLC (cell lines) and not in non-SCLC (cell cultures).\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10-1:100\nImmunohistochemistry (frozen sections): 1:10-1:100 using ABC detection\nWestern Blot: 1:50-1:500\nOptimum dilutions to be determined by the researcher.\n\nHybridoma: \nDerived by fusion of Sp2/0-Ag14 mouse myeloma cells with spleen cells from a Balb/c mouse. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2146-15A

Size

100ug

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, NHP, Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 40% glycerol. No stabilizing proteins added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1.

Additional Info

Recognizes an epitope located within the region of aa421-589 of the neuroendocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, this clone reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining. Species Crossreactivity: Human, rabbit and rhesus monkey.