Neuroendocrine Specific Protein C (NSP-C, Rtn 1C, Reticulon 1C)

Recently, a novel gene family has been identified and characterized, the protein products of which are localized in the endoplasmic reticulum. The proteins encoded by these genes were therefore named Reticulons. \nReticulon-1 (Rtn-1) was formerly designated NSP (Neuroendocrine-Specific-Protein), because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. NSP-A, NSP-B, and NSP-C. These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions.\nNSP-A is a protein with a molecular weight (MW) of about 135kD, which occurs in various isoforms presumably depending on the degree of phosphorylation in serine residues. In lung cancer diagnosis NSP-A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of NSP-A. NSP-B is a phosphoprotein with a MW of 45kD and is restricted to the lung cancer cell line NCI-H82. NSP-B is not found for so far in human tissues. NSP-C is a protein with a MW of 23kD which is not phosphorylated and is found with NSP-A in SCLC (cell lines) and not in non-SCLC (cell cultures). \n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50-1:100\nImmunohistochemistry: 1:50-1:100; Frozen tissue sections with avidin-biotinylated horseradish peroxidase complex (ABC) as the detection reagent.\nWestern Blot: 1:100-1:500\nOptimum dilutions to be determined by researcher.\n\nHybridoma:\nDerived by the fusion of SP2/0-Ag14 mouse myeloma cells, with spleen cells from a BALB/c mouse.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2146-25A

Size

100ug

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Senden, N.H., et al., Eur. J. Cell Biol. 69: 197-213 (1996). 2. Senden, N.H., et al., J. Pathol. 182: 13-21 (1997). 3. Hens, J., et al., Cell Tissue Res. 292: 229-237 (1998). 4. Kim, S.H., et al., Biochem. Biophys. Res. Commun. 276: 329-334 (2000).

Additional Info

Recognizes an epitope located within the first 20 amino acids of Neuroendocrine Specific Protein C (NSP-C). Reacts with peripheral nerves and ganglia of various tissues and crossreacts with smooth muscle cells and myoepithelium. In the central nervous system, reactis with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Species Crossreactivity: Human and rat.