Neurofilament H, 180-220kD (NF-H) )

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160 and 200kD) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70kD NF-L protein can self-assemble into a filamentous structure. The 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas, adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung express neurofilaments.\n\nApplications:\nSuitable for use in Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2160-06J

Size

100ul

Applications

ICC, IHC

Hosts

Mouse

Reactivities

Rat

Form

Ascites fluid, no preservatives

P Type

Mab

Purity

Ascites

Isotype

IgG

References

J. Neurocytology (1996) 25:181

Additional Info

Strong reactivity on rat NF-H (200kD), somewhat weaker on other mammalian species. Does not recognize avian NF-H. Staining is not affected by the level of NF-H phosphorylation.