Neurofilament H, 200kD, Bovine (NF-H)

Neurofilaments can be defined as the intermediate or 10nm filaments found specifically in neuronal cells. In the electron microscope neurofilaments appear as 10nm diameter fibers of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins known as NFL, NFM and NFH. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.\n\nIsoelectric Point:\npI 5.5\n\nApplications:\nSuitable for use as a Protein standard in 1D and 2D SDS gel electrophoresis, Immunoassays and for Immunization. Other applications not tested.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2160-15B

Size

100ug

Form

Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5, 2mM DTT, 6M Urea, 1mM EDTA, 10mM methylammonium chloride. Reconstitute with sterile ddH2O to a concentration of ~1mg/ml.

Purity

~98% (determined by SDS gel electrophoresis).

References

1. Dahl, D., Crosby, C.J., Gardner, E.E. and Bignami, A., Purification of the glial fibrillary acidic protein by anion-exchange chromatography. Analyt. Biochem. 126: 165 ff (1982). 2. Weber, K., Shaw, G., Osborn, M., Debus, E. and Geisler, N., Neurofilaments, a subclass of intermediate filaments: Structure and expression. Cold Spring Harbor Symp. Quant. Biol. 48: 717 ff (1983).