Neurofilament H, 200kD (NF-H)

Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200kD) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70kD NF-protein can self-assemble into a filamentous structure, whereas the 160kD and 200kD NF-proteins require the presence of the 70kD NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. The 200kD neurofilament protein is abundantly expressed in the spinal cord, cerebellum, pons and medulla. These are predominantly brain regions giving rise to long-axon neurons.\n\nAlthough IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes are NF proteins expressed together with desmin. In tumorpathology ganglioneuro-blastomas and some of the neuroblastomas are strongly positive with the neurofilament antisera. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.\n\nApplications:\nSuitable for use in Immunoblot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunoblot: 1:50-1:250\nImmunohistochemistry (frozen tissues): 1:25-1:50. Recommended Detecting Agent: Avidin-biotinylated horseradish peroxidase complex (ABC).\nOptimum dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2160-14A

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, NHP, Rabbit, Sheep, Xen/Amph

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Hens J, Nuydens R, Geerts H, Senden NHM, Ven WJM van de, Roebroek AJM, Velde HJK van de, Ramaekers FCS, Broers JLV - Neuronal differentiation is accompanied by NSP-C expression. Cell Tissue Res 292: 229-237 (1998) .\n\n2.

Additional Info

Reacts with the phosphorylated isoform of the 200kD bovine nerofilament protein. Species Crossreactivity: Human, rat, rabbit, canine, chicken, mouse, monkey, guinea pig, sheep, Xenopus.