Neurofilament, heavy chain, 180-220kD (NF-H)

Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight neurofilament subunit and runs on SDS-PAGE gels in the range 180-220kD with some variation in different species. This monoclonal recognizes specifically the phosphorylated form of NF-H which is normally found only in axons in the adult nervous system. Since closely related phosphorylated epitopes are also found on NF-M, this antibody shows some reactivity with phosphorylated forms of this protein also. Interestingly, in many damage and disease states the phosphorylated form of NF-H accumulate in the perikarya of neurons. The antibody can be used to identify axons in tissue sections and in tissue culture. It can also be used to study phosphorylated neurofilament accumulations seen in many neurological diseases and following neuronal injury.\n\nCan be used on cryostat sections and on formalin-fixed cells in tissue culture. The antibody works well in ELISA and Western Blot, and is particularly appropriate for paraffin-embedded histological sections.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N2160-06M

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Prc

Form

Supplied as ascites, 0.01M PBS, pH7.6, 0.05% sodium azide, 0.01% Thimerosal, 15mg/ml BSA

P Type

Mab

Purity

Ascities

Isotype

IgG1,k

References

1. Harris, J. et al. J. Neurosci. Res. 30:47-62 (1991).

Additional Info

Recognizes the phosphorylated variant of NF-H subunit (~180-220kD), showing some weaker reactivity with phosphorylated forms of NF-M. Species crossreactivity: Human NF-H and all other mammals tested to date. Also recognizes avian NF-H.