Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein)

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kD. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease and Alzheimer's disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (1).\n\nApplications:\nSuitable for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:100-1:500\nImmunohistochemistry: Frozen and mildly formalin-fixed sections\nWestern Blot: 1:5000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

031404

Size

100ul

Applications

ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Prc

Form

Supplied as a liquid.

P Type

Mab

Purity

Ascites

Isotype

IgG2b

References

1. Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46 (2000).

Additional Info

Recognizes NF-L from human, bovine, porcine, mouse, rat and all other mammals.