Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein)

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which include the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include other intermediate filamant subunits such as a-internexin, peripherin and in developing neurons nestin and vimentin. Antibodies to neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable and found only in neurons. Mutations in the protein coding region of the human NF-L gene appear to cause some forms of Charcot-Marie-Tooth disease (2) The HGNC name for this protein is NEFL.\n\nApplications:\nSuitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry (ABC): 1:5000\nImmunofluorescence: 1:500-1:1000\nWestern Blot: 1:20,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

031405

Size

100ul

Applications

ICC, IF, WB

Hosts

Rabbit

Reactivities

Rat, Prc

Form

Supplied as a liquid.

P Type

Pab

Purity

Serum

References

1. Delacourte A, Filliatreau G, Boutteau F, Biserte G and Schrevel J. Study of the 10-nm-filament fraction isolated during the standard microtubule preparation. Biochem J. 191:543-6 (1980).\n\n2. Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46 (2000).

Additional Info

Recognizes porcine and rat NF-L.