Neurofilament M, 145-160kD (NF-M)

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145,000~170,000 Da, with some variation in different species. Antibodies to NF-M are useful to identify this protein and identify neurons and their processes in tissue sections and in tissue culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. \n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:50\nWestern Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2160-01T1

Size

100ug

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Ch/Bird, Fel

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

References

1. Xia CH, et al. Abnormal neurofilament transport caused by targeted disruption of neuronal kinesin heavy chain KIF5A. J. Cell Biol. Apr 2003; 161(1): 55-66. 2. Lee MK, et al. Neurofilaments are obligate heteropolymers in vivo. J. Cell Biol. Sep 1993; 122(6): 1337-1350. 3. Pleasure SJ, et al. Pure, postmitotic, polarized human neurons derived from NTera 2 cells provide a system for expressing exogenous proteins in terminally differentiated neurons. J. Neurosci. May 1992; 12(5): 1802-1815.

Additional Info

Recognizes NF-M from human, rat, mouse, cat, chicken.