Neurofilament (NF, NF-H)

Neurofilaments (NFs), the intermediate filaments (IFs) specific to neurons, is the predominant cytoskeletal elements in many nerve cells. They are believed not only to confer mechanical stability, but also to play a role in determining axonal caliber. The human NF-H gene has three introns, two of which interrupt the protein-coding sequence at identical points to introns in the genes for the two smaller Neurofilament proteins, NF-M and NF-L having mol. wts predicted from their gene sequences of -110 000, -102 000 and -62 000 respectively. Each type of Neurofilament contains a central a-helical 'rod' domain of - 310aa, characterized by a heptad repeat in the amino acid sequence in which the first and fourth residues are apolar. Perikaryal NFs are predominantly unphosphorylated, while axonal NFs are highly phosphorylated. Defect in NFH causes susceptibility to amyotrophic lateral sclerosis. It has an important function in mature axons.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry(Paraffin): 1:200-1:400\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

138577

Size

20ug

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Bone cancer increases transient receptor potential vanilloid subfamily 1 expression within distinct subpopulations of dorsal root ganglion neurons. Niiyama Y, Kawamata T, Yamamoto J, Omote K, Namiki A. Neuroscience. 2007 Aug 24;148(2):560-72. Epub 2007 Jul 25. 2. Systemic polyethylene glycol promotes neurological recovery and tissue sparing in rats after cervical spinal cord injury. Baptiste DC, Austin JW, Zhao W, Nahirny A, Sugita S, Fehlings MG. J Neuropathol Exp Neurol. 2009 Jun;68(6):661-76. doi: 10.1097/NEN.0b013e3181a72605. 3. Mapping of Purkinje neuron loss and polyglucosan body accumulation in hereditary cerebellar degeneration in Scottish terriers. Urkasemsin G, Linder KE, Bell JS, de Lahunta A, Olby NJ. Vet Pathol. 2012 Sep;49(5):852-9. doi: 10.1177/0300985811412622. Epub 2011 Jul 13. 4. The retrograde delivery of adenovirus vector carrying the gene for brain-derived neurotrophic factor protects neurons and oligodendrocytes from apoptosis in the chronically compressed spinal cord of twy/twy mice. Uchida K, Nakajima H, Hirai T, Yayama T, Chen K, Guerrero AR, Johnson WE, Baba H. Spine (Phila Pa 1976). 2012 Dec 15;37(26):2125-35. doi: 10.1097/BRS.0b013e3182600ef7.

Additional Info

Recognizes neurofilament. Species Crossreactivity: human, rat