Neuroglobin

Neuroglobin, a 151 amino acid reside protein, mainly expressed in vertebrate brain and retina, is a recently identified member of the globin superfamily. Augmenting O2 supply, neuroglobin promotes survival of neurons upon hypoxic injury, potentially limiting brain damage. Moreover, neuroglobin may be a novel oxidative stress-responsive sensor for signal transduction in the brain. Neuroglobin expression is increased by neuronal hypoxia in vitro and focal cerebral ischemia in vivo, and neuronal survival after hypoxia is reduced by inhibiting neuroglobin expression with an antisense oligodeoxynucleotide and enhanced by neuroglobin overexpression.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nAmino Acid Sequence:\nMERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG E\n\nSuggested Secondary Antibodies: \nI1906: IgY, Chicken Pab Rb x Ch \nI1906-10: IgY, Chicken-HRP Rb x Ch \nI1906-12: IgY, Chicken-FITC Rb x Ch \nI1906-14: IgY, Chicken-AP Rb x Ch\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N1250-63

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Ch/Bird

Reactivities

Hum, Rat, Can

Form

Supplied as a lyophilized powder from PBS. Reconstitute with 50ul sterile ddH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgY

References

1. Li RC, Lee SK, Pouranfar F, Brittian KR, Clair HB, Row BW, Wang Y, Gozal D. Hypoxia differentially regulates the expression of neuroglobin and cytoglobin in rat brain. Brain Res . Jun 22;1096(1):173-9 (2006). 2. Ostojic J, Sakaguchi DS, de Lathouder Y, Hargrove MS, Trent JT 3, Kwon YH, Kardon RH, Kuehn MH, Betts DM, Grozdanic S. Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons. Invest Ophthalmol Vis Sci . Mar;47(3):1016-23 (2006). 3. Rajendram R, Rao NA. Neuroglobin in normal retina and retina from eyes with advanced glaucoma. Br J Ophthalmol . May;91(5):663-6 (2007)

Additional Info

Recognizes human Neuroglobin at ~17kD. \nSpecies Crossreactivity: canine and rat