NEUROLIGIN 3 (NLGN3, ASPGX1, AUTSX1, Gliotactin Homolog, HNL3, KIAA1480, Neuroligin-3, NL3)

NLGN3 is a 110-114kD member of the type-B carboxyesterase/lipase family of proteins. It is a neuronal transmembrane protein that forms Ca++-dependent intercellular junctions with short b-neurexin isoforms. This seems to contribute to both glutamatergic and GABAergic synapse formation. Mutations in NLGN3 are associated with a reduction in protein expression and the occurrence of autism. Mature human NLGN3 is an 811aa type I transmembrane protein. It contains a 672aa extracellular domain (ECD) aa38-709, plus a 118aa cytoplasmic region. The ECD possesses a nonfunctional carboxyesterase domain aa41-625 Multiple splice variants exist. There is a deletion of aa153-172 that may also be accompanied by an alternative start site at Met118, and a deletion of aa153-192 that may also be accompanied by a five Lys substitution for aa554-848. Over aa39-709, human NLGN3 shares 99aa identity with mouse NLGN3.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N1260-03D1

Size

100ug

Hosts

Mouse

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG2B

Additional Info

Recognizes human Neuroligin 3. Species sequence homology: Recombinant human Neuroligin 3v2 and recombinant rat Neuroligin 3v4 (100% ). No cross-reactivity with Neuroligin 2, Neuroligin 4, Neuroligin 1, 1v2, or 2v2.