Neuromedin U (NmU)

The neuromedins (Nm) are a family of bioactive peptides best known for their roles in smooth muscle contraction. Nm family of bioactive peptides include: Bombesin-like (NmB, NmC), kassinin-like (NmL and NmK or neurokinins A and B), neurotensin-like (NmN), and neuromedin U (NmU; for its ability to stimulate uterine muscle contraction). NmU-like immunoreactivity has been detected in mammalian brain, GI-tracts of various species, and in the thyroid and endocrine cells of pituitary glands. Besides its roles in smooth muscle contraction (human ileum, urinary bladder, rat stomach etc), NmU has also been implicated in hypertension, blood flow in intestine, and neurotransmission. Recently two structurally related, orphan G-protein coupled receptors, termed NMUR1 and NMUR2 have been identified as cognate receptors of NmU. \nNmU is synthesized from a large precursor peptide and cleaved into 25aa (human, 25aa; rat, 23aa; porcine, 25aa) and 8aa (Nmu-8; 18-25) biologically active peptides. NMU peptides from various species share the greatest homology in the their C-terminal regions, which is also critical in biological activity. NMU is present in nerves throughout the GI-tracts, corticotrophs within the anterior and lobe of rat and human pituitary glands, parafollicular cells of in rat thyroid gland, and in various regions of brain (spinal cord, hypothalamus, substantia nigra, hippocampus, amygdala). Low levels of NmU are also found in human adipose tissue, lymphocytes, and spleen.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:100,000 using 50-100ng of control peptide/well. See N2171-80G.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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