Neuromedin U (NmU)

The neuromedins (Nm) are a family of bioactive peptides best known for their roles in smooth muscle contraction. Nm family of bioactive peptides include: Bombesin-like (NmB, NmC), kassinin-like (NmL and NmK or neurokinins A and B), neurotensin-like (NmN), and neuromedin U (NmU; for its ability to stimulate uterine muscle contraction). NmU-like immunoreactivity has been detected in mammalian brain, GI-tracts of various species, and in the thyroid and endocrine cells of pituitary glands. Besides its roles in smooth muscle contraction (human ileum, urinary bladder, rat stomach etc), NmU has also been implicated in hypertension, blood flow in intestine, and neurotransmission. Recently two structurally related, orphan G-protein coupled receptors, termed NMUR1 (GPCR66/FM-3/SNORF62) and NMUR2 (TGR-1/FM-4/SNORF72), have been identified as cognate receptors of NmU. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml using ECL.\nELISA: ~1:100,000 using 50-100ng control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide: \nN2171-80N: Neuromedin U, Mouse Control Peptide (NmU)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2171-80P

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Lo G et al (1992) Mol. Endocrinol. 6, 1538-1544; 2. Minamino N et al (1988) BBRC 156, 355-360; 3. Conlon JM et al (1988) J. Neurochem. 51, 988-991; 4. Austin C et al (1994) J. Mol. Endocrinol. 14, 157; 5. Raddatz R et al (2000) J. Biol. Chem. 275, 32452; 6. Howard AD et al (2000) Nature 406, 70; 7. Fuji R et al (2000) JBC 275, 21068.

Additional Info

Recognizes mouse Neuromedin U. No significant sequence homology with rat or human or NmU from other species.