Neuron Cell Surface Antigen A2b5 (A2B5 Cell Surface Ganglioside) (Biotin)

Applications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution: \nFlow Cytometry: 0.1mg/ml. Add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of biotinylated monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml solution of a secondary developing reagent such as \nAvidin-Carboxyfluorescein or Streptavidin-Phycoerythrin.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nMouse myeloma with B cells obtained from a mouse immunized with embryonic chicken retinal \ncells.1. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2172A

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Ch/Bird

Conjugates

Biotin

Form

Supplied as a lyophilized powder from a 0.2 um filtered solution in PBS, containing 50ug BSA. Reconstitute with 40-50% glycerol, PBS.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgM

References

1. Eisenbarth, G.S. et al., 1979, Proc. Natl. Acad. Sci. USA 76:4913. 2. Bottenstein, J.E. et al., 1988, J. Neurosci. Res. 20:291. 3. Suzumura, A. and D.H. Slerberg, 1989, Brain Res. 480:51. 4. Dubois-Dalcq, M. et al., 1990, Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3 - 17.

Additional Info

Recognizes chicken. Species Cross Reactivity: human, rat, mouse.