Neuron Specific Enolase (NSE)

Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Perhaps not surprisingly since neurons require a great deal of energy they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2). The HGNC name for this protein is ENO2.\n\nApplications:\nSuitable for use in Immunofluorescence and Western Blot. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The NSE protein runs at about ~47kD on SDS-PAGE gels, and is a prominent component of brain, spinal cord and especially cortical extracts. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:500\nWestern Blot: 1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

031410

Size

100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 10mM sodium azide.

P Type

Pab

Purity

Serum

References

1. Shaw, G . Morse, S., Ararat, M. and Graham, F. Transformation of human cells by human adenoviruses and the origins of HEK 293 cells. Faseb Journal 16:869-871 (2002).\n\n2. Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. J. Neurotrauma 23:1201-1210 (2006).

Additional Info

Recognizes human and rat NSE.