Neuron-Specific Enolase (NSE) BioAssay(TM) ELISA Kit

The glycolytic enzyme enolase (2-phosph-D-glycerate hydrolyase) exists as several dimeric isoenzymes (aa, ab, ay and yy) composed of three distinct subunits, a, b, and y. Three isoenzymes are found in human brain: aa, ay and yy. The ay and yy-enolase isoenzymes are also known as neuron-specific enolase (NSE) as these isoenzymes initially were detected in neurons and neuronendocrine cells. The NSE levels are low in health and benign subjects. Elevated levels are commonly found in patients with malignant tumors with neuronendocrine differentiation, especially small cell lung cancer and neuroblastoma. Lung cancer is one of the most spread cancer forms with incidences about 50~100 per 100,000 population. Approximately 20% of the lung cancer is small cell lung cancer. Patients with small cell lung cancer show various proportions of ay and yy isoenzyme. The determination of NSE should detect and y isoforms with the same sensitivity (1). The antibodies for this particular assay are specific for the y-subunit without cross reactivity with a or b subunits(1). NSE Kits are reported to be useful diagnostic marker for lung cancer (2), neuroblastoma (3), and melanoma (4), seminoma (S) and in injury of central nervous system (6). In addition to the above, NSE can be a valuable tool in following-up the effect of chemotherapy of small cell lung cancer, in prognostic evaluation of patients with small cell lung cancer.\n\nIntended use:\nThe Neuron-Specific Enolase (NSE) enzyme linked immunosorbent assay (ELISA) provides quantitative measurement of human NSE in serum to aid in the clinical evaluation of patients suspected of having small cell lung cancer, and other related diseases.\ncer, and in differential diagnosis between cell lung cancer and non-small cell lung cancer.\n\nTest Principle\nThe NSE Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one monoclonal anti-gNSE antibody for solid phase (microtiter wells) immobilization and another monoclonal anti-gNSE antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The standards and test specimen (serum) are added to the antibody coated microtiter wells. During the incubation, specific NSE bound to anti-NSE antibody on the wells. Unbound NSE antigen is removed by washing the wells with buffer. Enzyme conjugate is then added to each well. After another incubation, unbound enzyme conjugate is washed off and the amount of bound peroxidase is proportional to the concentration of the NSE present in each sample. Upon addition of the substrate and chromogen, the intensity of blue color will develop in proportion to the concentration of NSE antigen in the samples.\n\nDetection Range: 0-200ng/ml\n\nSpecificity: 98.7%\n\nSensitivity: 1.5ng/ml\n\nKit Components: \nMicrotiter Plate, 1x96wells\nSample DIluent, 1x12ml\nEnzyme Conjugate Reagent, 1x12ml\nStandard 0ng/ml, 1x1vial\nStandard 5ng/ml, 1x1vial\nStandard 15ng/ml, 1x1vial\nStandard 40ng/ml, 1x1vial\nStandard 100ng/ml, 1x1vial\nStandard 200ng/ml, 1x1vial\nEnzyme Conjugate Reagent, 1x12ml\nTMB Substrate, 1x12ml\nStop Solution, 1x12ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

167562

Size

96Tests

Applications

ELISA

References

1. Paus E. Nustad K. Immunoradiometric assay for ag and gg-Enolase(Neuron Specific Enolase), with use of Monoclonal antibodies and Magnetizable Polymer Particles. Clin.Chem, 35:2034, 1989. 2. Paus E., Risberg T., Establishment and Evaluation of A Radioimmunoassy for Neuron-Specific Enolase, Tumour Biol 10:23-30, 1989. 3. Cooper E.H., Pritchard J, bailey CC, Serum Neuron-Specific Enolase in children's cancer. Br J Cancer. 56:65-67,1987.\n4. Wibe E., Paus E., Aamdal S., Neuron Specific Enolase (NSE) in serum of Patients with Malignant Melanoma, Cancer Letters, 52:29-31,1990.\n5. Fossa S.D., Kiepp O., Paus E., Neuron-Specific (NSE) a serum tumor marker in seminoma. Br. J. Cancer, 65, 297-299,1992. 6. Skogseid I.M., nordby H.K., Urdal P., Paus E., Lilleaas F., Increased serum creatine kinase BB and Neuron-Specific Enolase following Head Injury indicates brain damage, Acta Neuronchir(Wien) 115:106-111,1992. 7. Pahlman S., Esscher T., Bergvall p. and Odelstad L. Purification and Characterization of human - specific Enolase, Radioimmunoassay Development Tumor Biol. 5,127-139, 1984.