Neuron Specific Nuclear Protein (NeuN) (Biotin)

Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. \n\nApplications: \nSuitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: 1:100; Works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. Works well with formaldehyde- based fixatives. Citric acid and microwave pretreatment has been used successfully. Staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones.\nWestern Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD.\nImmunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1% triton X-100.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control: \nBrain tissue, most neuronal cell types throughout the adult nervous system\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2173-20

Size

500ug

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.1, 15mg/ml BSA, 0.1% sodium azide. Labeled with Biotin.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

US Biological application reference: L'Episcopo, F. et al., (2011) Neurobiology of Disease 41; 508-527. 1. Chera, et al. (2002) Ann. N.Y. Acad Sci. 965: 274-280. 2. Mullen, et al. (1992) Development 116: 201-211. 3. Wolf, et al. (1996) J. Histochem. Cytochem. 44:1167-1171. 4. Wood, G., Warnke, R. (1981). J. Histochem, Cytochem. 29: 1196-1204. 5. Moore, et al. (2009) Neuroscience. 164:1484-1495. 6. Mareschi, et al. (2009) Cytotherapy. 11: 534-47. 7. Hung, et al. (2009) Brain: a journal of neurology. 132:889-902. 8. Marianne, et al. (2009) Experimental brain research. Experimentelle Hirnforschung. Exp

Additional Info

Recognizes mouse Neuron Specific Nuclear Protein (NeuN). Reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Non-reactive cell types include Purkinje, mitral and photoreceptor cells. Species Crossreactivity: rat