Neuronal Marker, pan (Alexa Fluor(R)488)

Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques, such as Golgi staining, and modern molecular approaches, such as GFP constructs, yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, an Alexa 488(R) conjugated version of the pan-neuronal antibody blend was created to facilitate immunofluorescence studies. Each antibody within the blend is conjugated to Alexa 488(R) therefore enabling detection of an entire neuron with only one microscopic emission channel. This antibody mixture has been validated in diverse fixations, cell culture, and immunohistochemistry protocols, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology. High morphological resolution has been achieved, including distral dentrites and spines.\n\nApplications:\nSuitable for use in Immunocytochemistry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nImmunocytochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nCortical neurons\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degrees C. Freezing Alexa Fluor(R) conjugates will result in a substantial loss of activity. Alexa Fluor(R) conjugates are sensitive to light.

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