Neuronal Marker, Pan (Cy3)

Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques such as Golgi staining and modern molecular approaches such as GFP constructs yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, this polyclonal antibody blend reacts against key somatic, nuclear, dendritic, and axonal proteins distributed across the pan-neuronal architecture that can then be detected by a single secondary antibody. This antibody cocktail has been validated in diverse methods, cell culture and immuno-histochemistry, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology.\n\nApplications:\nSuitable for use in Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRat E18 cortex primary neuron cell culture or mouse adult brain cryosections. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Cy conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

029559

Size

100ul

Applications

ICC, IF, IHC

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, 15mg/ml BSA, 0.05% sodium azide. Labeled with Cy3.

P Type

Pab

Purity

Purified

References

1. Long, K. et al. (2008) Abstract; Society for Neuroscience Annual Meeting. 2. Speckmann, W. et al. (2011) Abstract; Society for Neuroscience Annual Meeting.

Additional Info

Recognizes axons, dendrites, nuclei, and the cell body (soma) of neurons. Species Crossreactivity: rat and mouse.