Neuropeptide Y (NPY)

Neuropeptide Y (NPY), a neuropeptide colocalized and abundantly released with the neurotransmitter norepinephrine in nervous system, synthesized by postnatal olfactory epithelium of sustentacular cells. It promotes the proliferation of postnatal neuronal precursor cells and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion and potent vasoactive effects on the cardiovascular system. NPY precursor is a 97aa peptide in mouse, human (chr 7p15.1) and 98aa in rat has a MW of 11kD. The precursor contains a 28aa signal peptide, 29-64 (active processed NPY peptide) followed by the C-flanking peptide of NPY. NPY shows sequence homology to Peptide YY (PYY) and over 50% homology in aa and nucleotide sequence to Pancreatic Polypeptide (PNP or PPY), all of these homologous peptides has a 36aa mature peptide characterized by a hairpin loop.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml\nWestern Blot: 1-10ug/ml. Using ECL technique.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nN2176-60L: Neuropeptide Y, Mouse, Control Peptide \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2176-60K

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hansel D. E et al (2001) Nature 410: 940-943; 2. Thiele, T. E et al (1998) Nature 396: 366-369; 3. Maccarrone C. D et al (1986) Neurochem. Int 8: 13-22; 4. Gerald FDiBona (2002) Am J. Physiol Regulatory Integrative Comp Physiol 282: 635-36 (review).

Additional Info

Recognizes mouse Neuropeptide Y. Species sequence homology: rat, human and chicken (100%).