Neuropilin 1 (Neuropilin-1 Transmembrane Receptor, NP1, NRP, NRP 1, NRP1, NRP-1, BDCA4, CD304, DKFZp686A03134, DKFZp781F1414, NEL-like Protein 1, Nel-related Protein 1, OTTHUMP00000020818, OTTHUMP00000020820, OTTHUMP00000020821, Vascular Endothelial Cell

Neuropilin-1 is a type 1 membrane protein with three distinct functions. First, it can mediate cell adhesion via a heterophilic molecular interaction. Second, in neuronal cells, neurophilin-1 binds the class 3 semaphorins, which are neuronal chemorepellents, and plays a role in the directional \nguidance of axons.NP-1 isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis and in the formation of certain neuronal circuits and in organogenesis outside the nervous system. The soluble NP-1 isoform 2 binds VEGF(165) and appears to inhibit its binding to cells. It may also induce apoptosis by \nsequestering VEGF(165). NP-1 may bind various members of the semaphorin family. Its expression has an adverse effect on blood vessel number and integrity. \n \nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:50\nWestern Blot: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (paraffin): 1:100 \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman placenta, HUVEC, HepG2 cell lysates, mouse heart tissue, mouse kidney tissue, Rat heart lysate, rat kidney tissue, human kidney tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N2176-69

Size

100ul

Applications

FC, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Gluzman-Poltorak Z, et al. J Biol Chem 275(24):18040-5, 2000. 2. Shintani Y, et al. EMBO 25(13):3045-55, 2006. 3. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008).

Additional Info

Recognizes human Neuropilin 1 at ~130kD. Species Crossreactivity: mouse, rat, monkey