Neuropilin-1 (NRP1, BDCA4, CD304, DKFZp686A03134, DKFZp781F1414, NP1, NRP, Vascular endothelial cell growth factor 165 receptor, VEGF 165R) (Carboxyfluorescein)

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted Semaphorin subfamily as well as some isoforms of VEGF family proteins. The aa sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively. \n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated on bEND.3 cells and PC-12 cells.\nOptimal dilutions to be determined by the researcher.\n\nFlow Cytometry Protocol: \n1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. \n2. After blocking, 10ul of N1276-91C was added to 1-2.5x10e5 cells and incubated for 30 minutes at RT.\n3. Unbound antibody was removed by washing the cells \ntwice in Flow Cytometry Staining Buffer (Catalog #F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.\n4. The cells were resuspended in Flow Cytometry Staining \nBuffer for analysis by Flow Cytometry. As a control for this \nanalysis, cells in a separate tube should be treated with \nCFS-labeled goat IgG antibody. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Carboxyfluorescein conjugates are sensitive to light.

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