Neurotensin Receptor 1 (NTR1)

Neurotensin is an endogenous tridecapeptide neurotransmitter that triggers hypothermic and naloxone-insensitive analgesic responses, whereas peripheral effects include hypotension, decrease gastric acid release, and potentiation of lipid digestion. NT causes contraction of smooth muscle. NT is widely distributed throughout the central nervous system. NT gene (human chromosome 12q21) produces both NT and neuromedin N (NN). NT initiates its biological action by interacting with two distinct G-protein coupled receptors (NTR1 and NTR2). Recently, a third receptor, NTR3, has been identified that is identical to gp95/sortilin and it is not coupled via the G-proteins. All three receptors bind NT through its C-terminal hexapeptide sequence (RRPYIL, aa 8-13). Biologically active NTT (NT 8-13) has also been shown to interact with the extracellular domain 3 (between TM 6-7) of NTR1.\n\nNTR1 is the levocabastine-insensitive, high affinity receptor for NT. It has the typical structure of GPCR: 7 TM domains with extracellular N-terminus and cytoplasmic C-terminus. It has been shown to mediate a number of peripheral and central NT responses, including the neuroleptic-like effects of the peptide. It is highly expresssed in the brain, small intestine and other peripheral tissues.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL. NTR1 has been shown to be 52-54kD in rat brain. NTR1 may be differentially glycosylated.\nELISA: 1:100,000 using 50-100ng control peptide/well. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2177-24

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.

Additional Info

Recognizes the human Neurotensin Receptor 1 (NTR1). Species Sequence Homology (72%): mouse and rat. No significant sequence homology exist with NTR2 or NTR3 or other GPCR.