Neurotensin Receptor 3 (NTR3, Sortilin)

Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter that influences distinct central and peripheral physiological functions in mammals. Central administration of NT modulates dopaminergic transmission and triggers hypothermic and naloxone-insensitive analgesic responses, whereas peripheral effects include hypotension, decrease gastric acid release, potentiation of lipid digestion. NT is widely distributed throughout the CNS. It has been localized to catecholamine-containing neurons. NT initiates its biological action by interacting with two distinct G-protein coupled receptors (NTR1 and NTR2). Recently, a third receptor NTR3 has been identified that is identical to gp95/sortilin and it is not coupled via the G-proteins. All three receptors bind NT through its C-terminal hexapeptide sequence (8 RRPYIL 13). Biologically active NT (NT8-13) has also been shown to interact with the extracellular domain 3 (between TM6-7) of NTR1.\nMost recently, a novel, non-GPCR, NTR3/sortilin/gp95/NT3 \n(mouse 825 aa, rat 748 aa; human 833 aa, chromosome 1p21.3; ~92% identity between human and mouse) also binds NT. Sortilin was initially isolated as by its affinity for receptor-associated protein (RAP). NTR3, the first non-GPCR neuropeptides receptor, sequence includes a putative secretory signal peptide, a furin cleavage site, and a single transmembrane domain near the C-terminus. The mature protein has 44 N-terminal residues removed during processing. It is homologous to the yeast protein Vps10p and the human sortilin-related receptor. The C terminus of the protein contains a lysosomal sorting motif shared by the mannose-6-phosphate receptors. NTR3 is expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta, and testis.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL.\nELISA: 1:100,000 using 50-100ng control peptide/well.\nOptimal dilutions to be determined by the researcher.\nControl Peptide: N2177-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2177-52

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by affnity chromatography.

Isotype

IgG

References

References\n(1) Peterson Cm et al (1997) JBC 272, 3599; Mazella J et al (1998) JBC 273, 26273; Morris NJ et al (1998) JBC 273, 3582; Lin BZ et al (1997) JBC 272, 24145; Nielsen MS et al (1999) JBC 274, 8832

Additional Info

Recognizes human Neurotensin Receptor 3. Species sequence homology: mouse and rat NTR3: 94%. No significant sequence homology exists with NTR1/NTR2 or other GPCR.