Neurotrophin-3 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse Neurotrophin-3 in cell culture supernates and serum.\n\nNeurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo.4 NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 2 pg/ml\n\nCrossreactivity:\nThere is cross-reactivates with NT-4 <2.5%, and no detectable cross-reactivity with any other cytokine.\n\nUniprot ID:\nP20181\n\nEntrez Gene ID:\n18205\n\nKit Components:\n96-well plate pre-coated with anti-Neurotrophin-3, Mouse\nLyophilized recombinant Neurotrophin-3, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143990

Size

96Tests

References

1. Ozcelik, T.; Rosenthal, A.; Francke, U. Chromosomal mapping of brain-derived neurotrophic factor and neurotrophin-3 genes in man and mouse. Genomics 10: 569-575, 1991. \n2. Tessarollo, L.; Vogel, K. S.; Palko, M. E.; Reid, S. W.; Parada, L. F. Targeted mutation in the neurotrophin-3 gene results in loss of muscle sensory neurons. Proc. Nat. Acad. Sci. 91: 11844-11848, 1994. \n3. Donovan, M. J.; Hahn, R.; Tessarollo, L.; Hempstead, B. L. Identification of an essential nonneuronal function of neurotrophin 3 in mammalian cardiac development. Nature Genet. 14: 210-213, 1996. \n4. Kalcheim, C.; Carmeli, C.; Rosenthal, A. Neurotrophin 3 is a mitogen for cultured neural crest cells. Proc. Nat. Acad. Sci. 89: 1661-1665, 1992. \n