Neurotrophin Receptor, p75

The Low Affinity Nerve Growth Factor Receptor (also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors which stimulate neuronal cells to survive and differentiate. The neurotrophins are comprised of four proteins, all of which bind to the LNGFR: Nerve Growth Factor (NGF), Brain Derived Neurotrophic Factor (BDNF), Neurotrophin-3 (NT-3), and Neurotrophin-4 (NT-4).\nNerve growth factor, the prototypical growth factor, is a protein secreted by a neuron's target. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. The NGF/TrkA complex is subsequently trafficked back to the cell body. This movement of NGF from axon tip to soma is thought to be involved in the long-distance signaling of neurons.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-05ug/ml detects p75 neurotrphin receptor in RIPA lysates from PC-12 cells. PC-12 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with N2017-42 (0.1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.\nImmunoprecipitation: Reported to immunoprecipitate p75 neurotrophin receptor.\nImmunohistochemistry: Reported to detect p75 neurotrophin receptor in rat brain sections (3).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2017-42

Size

100ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. von Schack, D., et al., Nat. Neurosci. 4: 977-978 (2001). 2. Salehi, A.H., et al., Neuron 27: 279-288 (2000). 3. Roux, P., et al., J. Neurosci. 19: 6887-6896 (1999).

Additional Info

Recognizes rat p75 neurotrophin receptor, Mr ~75kD.\nSpecies Crossreactivity: human and mouse.