Neurotrypsin (Leydin, Motopsin)

Neurotrypsin is a central nervous system-expressed serine protease whose truncation or absence causes nonsyndromic mental retardation (1-3). It is most prominently expressed in structures that are involved in the processing and storage of learned behavior and memory, such as the cerebral cortex, the hippocampus, and amygdala (1). Evidence suggests that neurotrypsin has multiple functions, including axonal outgrowth, maintaining neuronal plasticity, and arranging the perineuronal environment, partly in coordination with other proteases including tissue plasminogen activator (3). At least two isoforms of neurotrypsin are known to exist.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1 ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSK-N-SH cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N1225-01

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Gschwend TP, Krueger SR, Kozlov SV, et al. Neurotrypsin, a novel multidomain serine protease expressed in the nervous system. Mol. Cell. Neurosci. 1997; 9:207-19. 2. Molinari F, Rio M, Meskanaite V, et al. Truncating neurotrypsin mutation in autosomal recessive nonsyndromicmental retardation. Science 2002; 1779-81. 3. Mitsui S, Yamaguchi N, Osako Y, et al. Enzymatic properties and localization of motopsin (PRSS12), a protease who absence causes mental retardation. Brain Res. 2007; 1136:1-12.

Additional Info

Recognizes human neurotrypsin. Species Crossreactivity: Mouse and rat.