logo
logo
Neutrophils, Apoptotic

Cat no: N5201

Neutrophils, Apoptotic

Neutrophils are recruited in large numbers to inflammation sites. Clearance of these cells from the inflammatory site is done by programmed cell death (apoptosis) of the neutrophil. As a consequence, many cellular mechanisms are down-regulated in the apoptotic neutrophil such as: adhesion, chemotaxis, phagocytosis, enzyme secretion, generation of a respiratory burst, and responsiveness to external stimuli. Changes in the cell surface of dying neutrophils are recognized by macrophages, which destroy the neutrophils by phagocytosis. Many changes in the amount of proteins, carbohydrates and lipids on the surface of neutrophils are associated with the apoptotic event. Apoptotic Neutrophils (BOB78) is an up-regulated protein, molecular weight 90 kDa, that appears in the granular compartment of the late apoptotic neutrophil. This antigen does not appear in freshly isolated erythrocytes, platelets, lymphocytes, monocytes, or neutrophils. Monoclonal antibodies specific for BOB78 are a useful tool for studying the biology of apoptotic neutrophils.\n\nApplications: \nSuitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. \n\nRecommended Dilution:\nFlow Cytometry: 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTHP-1 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N5201

Size

100ug

Applications

FC, ICC, IHC, IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1M Tris-Glycine, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgM,k

Additional Info

Recognizes human Apoptotic Neutrophils.

Read more on Supplier website
Advertisehere
MtoZ Horizontal banner
MtoZ Biolabs

Latest promotion FROM:

MtoZ Biolabs
View promotion

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...