Newcastle Disease Virus (NDV)

Newcastle disease is a highly contagious disease affecting wild and domestic birds.Symptoms of the disease vary greatly, depending on the strain of virus and the species, age and health of the bird. Virulence of the disease can also vary greatly, with some strains causing very high mortality rates. In humans, NDV can cause mild influenza-like symptoms. The HN protein of the virus is a type II membrane glycoprotein on virion and infected cell surfaces. It binds to sialic acid-containing cellular receptors and promotes activity of the viral fusion protein, thereby allowing the virus to penetrate the cell. It also removes sialic acids from progeny virus particles to prevent viral self-agglutination. Newcastle disease virus is tumor selective and oncolytic. It has shown promise for selective growth in and killing of a variety of tumor cells.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2275-01C

Size

200ug

Applications

ELISA

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Lorence, R.M. et al. (2007) Phase 1 clinical experience using intravenous administration of PV701, an oncolytic Newcastle disease virus. Curr. Cancer Drug Targets. 7: 157-67.2. Huang Z. et al. (2003) Recombinant Newcastle disease virus as a vaccine vector. Poult. Sci. 82: 899-906.

Additional Info

Recognizes the hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV).