NF-kB p65 (Nuclear Factor kB)

The NF-kB family of induced-activated transcription factors are dimers comprised of members of the NF-kB /rel protein family. Activation of NF-kB is induced by a remarkably large number of stimuli including viruses, TNF, IL-1, PMA , LPS, UV light, and others. In most cell types, NF-kB is present in the cytoplasm as a 50kD (p50) subunit and a 65kD (p65, rel A) subunit. In unstimulated cells, nuclear localization signals present on p65 are masked by members of the Ik-B family of inhibitory proteins. Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosome-dependent pathway, allowing nuclear translocation of the active dimeric NF-kB transcription factor. In the nucleus, NF-kB binds to consensus sequences where, along with other co-factors, it participates in activating or enhancing the expression of specific genes.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Gel Mobility Shift Assay. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1.0ug/ml \nWestern Blot: 1ug/ml \nGel Mobility Shift Assay: 5-10ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N2303-05

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

Verma, I.M., et al., Genes Dev. 9:2723

Additional Info

Specific for NF-kB (p65). Crossreactivity with related proteins has not been observed. Species Sequence Homology: Based on amino acid homology, reactivity with mouse and rat NFkB is likely.