NF-kB p65, phosphorylated (Ser536) (Nuclear Factor kB) (AlexaFluor(R) 488)

Transcription factors of the nuclear factor kB (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals; RelA, c-Rel, RelB, NF-kB1 p105/p50), and NF-kB2 (p100/p52). p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. The p50 and p52 products\nform dimeric complexes with Rel proteins, which are then able to bind DNA and regulate transcription. In unstimulated cells, NF-kB is sequestered in the cytoplasm by its inhibitory proteins, the IkBs. NF-kBactivating agents can induce the phosphorylation of IkBs, which targets them for rapid degradation through a ubiquitin-proteasome pathway, releasing NF-kB to enter the nucleus and regulate gene expression. Processing of NF-kB2 is regulated by IKK1 (IKK-a), which triggers the phosphorylation and processing to p52, which can then undergo nuclear translocation.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10. Add 10ul of the conjugated antibody to 500K cells in 90ul PBS/0.5% BSA.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nStore at 4 degrees C before opening. DO NOT FREEZE. This product is stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Freezing Alexa Fluor(R)488 conjugates will result in a substantial loss of enzymatic activity.

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SPECIFICATIONS

Catalog Number

N2303-05A

Size

500ul

Applications

FC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% Sodium azide, 2.0 mg/ml BSA. Labeled with AelxaFluor 488.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Baeuerle, P.A. and Henkel, T. (1994) Annu. Rev. Immunol. 12: 141

Additional Info

Detects human NF-kB p65 only when phosphorylated at serine 536. Does not cross-react with the p50 subunit or other related proteins. Species Sequence Homology: mouse and rat: 100%.