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NFAT2, aa428-716 (NFATC1, Nuclear Factor of Activated T-cells)

Cat no: N2375-10B

NFAT2, aa428-716 (NFATC1, Nuclear Factor of Activated T-cells)

Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1(NFATc1) is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:2000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nMouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NFAT2 protein.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2375-10B

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2

References

1. Akimzhanov A, et al., (2008) Am J Pathol 172(1):215-224. 2. Asagiri M, et al., (2005) J Exp Med 202(9):1261-1269. 3. Rao A, et al., (1997) Annu Rev Immunol 15:707-747

Additional Info

Recognizes human NFAT2.

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