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NFAT5 (Nuclear Factor of Activated T-cells)

Cat no: N2375-35

NFAT5 (Nuclear Factor of Activated T-cells)

The Nuclear Factor of Activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. \nFour NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT 1 (also known as NFAT p or NFAT c2), NFAT 2 (NFAT c or NFAT c1), NFAT 3, and NFAT 4(NFAT x or NFAT c3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT 5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent. \n\nApplications: \nSuitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Gel Supershift assays. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000; Detects an ~170kD protein representing NFAT 5 in HEK293 cells transfected with the human NFAT 5 gene. \nImmunofluorescence (IC): 1:100-1:1000; Immunocytochemical staining of NFAT 5 in HEK293 cells transfected with the human NFAT5 gene results in primarily cytoplasmic staining. \nGel Supershift Assays: 1:1-1:1000\nImmunoprecipitation: 3ug\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2375-35

Size

50ug

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Nuclear Factor of Activated T-cells 5 (NFAT 5).

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