NFkB Activating Kinase (Nuclear Factor kappa B, TANK-Binding Kinase 1, NAK, TBK1)

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recently, a new IKK family member, TBK1 (TANK-binding kinase) has been identified (8). TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2301-40B

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Verma, I.M., Stevenson, J.K., Schwarz, EM., Van Antwerp, D. & Miyamoto, S. Genes. Dev. 9: 2723-2735 (1995). 2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci. USA 94: 11758 (1997). 3. Didonato, J.A., Hayakawa, M., Rothwarf, D.M., Zandi, E., and Karin, M. Nature 388: 548- (1997). 4. Regnier, C.H., Ho, Y.S., Gao, X., Goeddel, D.V., Cao, Z., and Rothe, M. Cell 90, 373 (1997). 5. Mercurio, F., Zhu, H., Murray, B.W., Shevchenko, A., Bennett, B.L., Li, J., Young, D.B., Barbosa, M., Mann, M. ,Manning, A., Rao, A. Science 278: 860 (1997). 6. Malinin, N.L., Boldin, M.P., Kovalenko, A.V., and Wallach, D. Nature 385: 540-544 (1997). 7. Lee, F.S., Hagler, J., Chen, J., and Maniatis, T. Cell 88: 213-222 (1997). 8. Pomeerantz, J.L. and Baltimore, D. EMBO J 18: 6694-6704 (1999). 9. Katherine A. Fitzgerald et al., IKKe and TBK1 are essential components of the IRF3 signaling pathway. Published online: 14 April 2003, doi:10.1038/ni921

Additional Info

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recently, a new IKK family member, TBK1 (TANK-binding kinase) has been identified (8). TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C or colder. Aliquots are stable for at least 24 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.