NFkB, p50 (NF-kB, Nuclear Factor kappa B p50 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B cells 1, NFkB1, DKFZp686C01211, DNA Binding Factor KBF1, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kB, Nuclear Factor kappa B DNA Binding Subuni

The NFkB family of induced-activated transcription factors are dimers comprised of members of the NFkB /rel protein family. Activation of NFkB is induced by a remarkably large number of stimuli including viruses, TNF, IL-1, PMA , LPS, UV light, and others. In most cell types, NFkB is present in the cytoplasm as a 50kD (p50) subunit and a 65kD (p65, rel A) subunit. In unstimulated cells, nuclear localization signals present on p65 are masked by members of the IkB family of inhibitory proteins. Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosomedependant pathway, allowing nuclear translocation of the active dimeric NFkB transcription factor. In the nucleus, NFkB binds to consensus sequences where, along with other co-factors, it participates in activating or enhancing the expression of specific genes. \n\nApplications: \nSuitable for use in ELISA, Western Blot and Gel Mobility Shift Assay. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1.0ug/ml \nWestern Blot: 1ug/ml \nGel Mobility Shift Assay: 5-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2303-10

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Verma, I.M., et al., Genes Dev. 9:2723

Additional Info

Recognizes human NFkB (p50). Crossreactivity with related proteins has not been observed. Reactivity with NF-kB (p50) was confirmed by immunoblot analysis of extracts of A431 cells. Reactivity with NF-kB (p50) was confirmed by immunoblot analysis of extracts of human A431cells. Species sequence homology: Mouse, rat and chicken