NFkB, p50, p105 (NF-kB, Nuclear Factor kappa B p50 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B cells 1, NFkB1, DKFZp686C01211, DNA Binding Factor KBF1, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kB, Nuclear Factor kappa B DNA Binding

Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB (2). Association with inhibitory proteins of the IkB family retains NF-kB in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (3). The p105 precursor protein acts as an NFkB inhibitor, retaining Rel subunits in the cytoplasm of unstimulated cells. Upon stimulation by TNFa and IL -1a, degradation of p105 occurs; the Rel subunit is released and translocats to the nucleus (4). Additionally, p105 will be proteolytically processed by the 26S proteasome to produce a smaller, transcriptionally active form (p50) (5).\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:30\nWestern Blot: 1:5,000\nImmunohistochemistry: 1:250-500\nImmunocytochemistry: 1:250-500\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N2302-01F

Size

100ul

Applications

FC, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Baldwin , A. S., Jr. (1996) Annu. Rev. Immunol. 14, 649-683 2. Verma, I., Stevenson, J., Schwarz, E., Van Antwerp, D. & Miyamoto, S. (1995) Genes Dev. 9, 2723-2735 3. Marc D. Jacobs Stephen C. Harrison. (1198); Cell Volume 95, Issue 6, 749-758 4. Rice, N. R., MacKichan, M. L., and Israel, A. (1992) Cell 71, 243-253 5. Palombella, V. J., Rando, O. J., Goldberg, A. L., and Maniatis, T. (1994) Cell 78, 773-785

Additional Info

Recognizes NF-kB p50 subunit