NFkB p65, acetaldehyde (Lys310) (NF-kB p65, NF-kB, Transcription Factors of the Nuclear Factor kB)

Transcription factors of the nuclear factor k B (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-kB1 (p105/p50), and NF-kB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-kB is sequestered in the cytoplasm by IkB inhibitory proteins (3-5). NF-kB-activating agents can induce the phosphorylation of IkB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKa (IKK1) regulate the phosphorylation and processing of NF-kB2 (p100) to produce p52, which translocates to the nucleus (9-11).\nNF-kB assembly with IkB, DNA binding, and transcriptional activity, are regulated by p300/CBP acetytransferases that principally target Lys218, Lys221, and Lys310 (12-14). This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-kB (12-14).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137473

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. 2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. 3. Haskill, S. et al. (1991) Cell 65, 1281-9. 4. Thompson, J.E. et al. (1995) Cell 80, 573-82. 5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. 6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. 8. Chen, Z.J. et al. (1996) Cell 84, 853-62. 9. Senftleben, U. et al. (2001) Science 293, 1495-9. 10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85. 11. Xiao, G. et al. (2001) Mol Cell 7, 401-9. 12. Ashburner, B.P. et al. (2001) Mol Cell Biol 21, 7065-77. 13. Mayo, M.W. et al. (2003) J Biol Chem 278, 18980-9. 14. Chen, L.F. et al. (2002) EMBO J 21, 6539-48.

Additional Info

Recognizes overexpressed levels of human NF-kB p65 when acetylated at Lys310. Species Crossreactivity: mouse