NFkB, p65, ID (NF-kB, Nuclear Factor kappa B, MGC131774, NFKB3, Nuclear Factor NF-Kappa-B p65, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells, RelA, Transcription Factor p65, v-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A)

NFkB is a heterodimer consisting of a 50kD DNA binding subunit and a 65kD transactivating subunit (RelA). The p50/p65 heterodime is present in the cytoplasm in inactive form bound to its inhibitor, IkB. In unstimulated cells, nuclear localization signals present on p65 are masked by members of the IkB family of inhibitory proteins. Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosome- dependent pathway, allowing nuclear translocation of the active dimeric NFkB transcription factor.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 3-5ug/extract from 10e7 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2302-02Z2

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG

References

1. Seiji Mabuchi, et al. J. Biol. Chem., 10.1074 (2004). 2. Romashkova JA, Makarov SS Nature. 401 86 (1999). 3. Nolan GP Cell 64 961(1991). 4. Lentsch A Immunol Ther Exp 48 59 (2000). 5. Hatada EN et al. Curr Opin Immunol 12:52 (2000).

Additional Info

Recognizes human NFkB p65.