NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA) (Biotin)

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml A band of ~60kD should be detected\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Controls:\nRamos, Daudi, HeLa and NIH 3T3 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2303-01B

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Nolan GP, et al. Cell 64(5):961-9 (1991). 2. Ruben SM, et al. Science 251(5000):1490-3 (1991). 3. Ganchi PA, et al. Mol Biol Cell 3(12):1339-52 (1992). 4. Toledano MB, et al. Mol Cell Biol 13(2):852-60 (1993).

Additional Info

Recognizes human NFkB. NF-kB (nuclear factor kB). Species Crossreactivity: Mouse