NFkB, p65, NLS Specific (NF-kB, Nuclear Factor kappa B, RelA)

NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as aheterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding IkB-a binding masks the NFkB nuclear localisation signal (NLS).\n\nApplications: \nSuitable for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and Supershift assays. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunohistochemistry: 1:100\nImmunofluorescence microscopy: 1:100\nSuper shift: 0.5-1ul/assay.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2303-02

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2 0.1% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Delfino, F. and Walker, W.H., Molecular and Cellular Endocrinology 157:1-9.(1999). 2.Zhang G, and Ghosh S. J Endotoxin Res 6(6): 453-7.(2000). 3.Unlap, M.T. and Jope, R.S. .Molecular Brain Research 45: 83-89.(1997). 4. Zabel U, et al.Nuclear uptake control of NF-kappa B by MAD-3, an I kappa B protein present in the nucleus. EMBO 12:1, 201-211. (1993). 5. Sen, R. and Baltimore, D. Cell 46:5, 705-716. (1986). 6. Lenardo, M.J. and Baltimore, D., Cell 58:2, 227-229. (1989). 7. Kawakami, K. et al., Proc Natl Acad Sci U S A 85:13, 4700-4704.(1988) 8. Baeuerle, P.A. and Baltimore D. Cell 53:2, 211-217. (1988). 9. Lenardo, M., Pierce, J.W. and Baltimore, D. Science 236:4808, 1573-1577.(1987).

Additional Info

Recognizes the nuclear localization sequence (NLS) NLS of human p65. The epitope overlaps the NLS of the p65 subunit of the NFkB heterodimer.