NFkB, p65 (Transcription Factor p65, Nuclear Factor NF-kappa-B p65 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-cells 3, NFKB3, RELA)

RelA belongs to a family of transcription factors (NFKB) that play a fundamental role in inflammatory and immune responses. The NFKB complex is composed of a heterodimer of a Rel family member (RelA, c-Rel, RelB) and either NFKB1 or NFKB2 subunits. RelA and NFKB1 are the most common heterodimeric pair. The NFKB complex is sequestered in the cytoplasm by inhibitory IKB proteins. Upon cellular activation, the ubiquitin-proteosome pathway degrades the IKB proteins allowing the NFKB complex to translocate to the nucleus and activate gene transcription.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunoprecipitation: 5ug labels 5 x10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts