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NFkB2 (Nuclear Factor kappa B)

Cat no: N2301-39B

NFkB2 (Nuclear Factor kappa B)

Nuclear Factor kappa B2 (NFkB2 or NFkB p52) is a member of the NFkB/Rel family of transcription factors. NFkB2 dimerizes with other members of the NFkB/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.\n\nHybridoma: \nProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived recombinant human Nuclear Factor kappa B2 (rhNFkB2; aa 1-447: Accession # NP_002493).\n\nApplications:\nSuitable for use in ChIP, Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nChromatin Immunoprecipitation (ChIP): 5ug per 5x10e6 cells\nImmunocytochemistry: 8-25ug/ml\nWestern Blot: 0.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2301-39B

Size

100ug

Applications

ICC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a powder lyophilized from a sterile-filtered solution in PBS containing 5% trehalose. Reconstitute to 0.5mg/ml with sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Detects human NFkB2. Does not detect mouse NFkB2 in NIH 3T3 cells.

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