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NGF-beta, Recombinant, Human, (Human NGF-beta, NGF-beta, NGFbeta, NGF beta, h-NGF-beta, rh-NGF-beta, recombinant human NGF-beta, recombinant NGF-beta, NGF)

Cat no: 167921

NGF-beta, Recombinant, Human, (Human NGF-beta, NGF-beta, NGFbeta, NGF beta, h-NGF-beta, rh-NGF-beta, recombinant human NGF-beta, recombinant NGF-beta, NGF)

Nerve growth factor-b (NGF-b) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-b is a dimer, formed by two identical 119aa subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 119aa and having a molecular weight of 16.95kD. The protein is purified by proprietary chromatographic techniques.\n\nSource:\nRecombinant corresponding to human NGF-beta expressed in CHO cells.\n\nMolecular Weight:\n-17kD\n\nBiological Activity:\nThe ED50 as determined using PC12 cell line is 1.2-2 ng/ml and using DRG cell line is 0.8-1.5ng/ml.\n\nEndotoxin:\n~0.1ng/ug\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

167921

Size

20ug

Form

Supplied as a lyophilized powder in 30mM sodium acetate, pH 5.5, 1% HAS. Reconstitute in dH2O to 0.1-1.0mg/ml after centrifugation.

Purity

~98% (HPLC, SDS-PAGE)

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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