Nerve growth factor-b (NGF-b) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-b is a dimer, formed by two identical 119aa subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing two identical 119aa and having a molecular weight of 16.95kD. The protein is purified by proprietary chromatographic techniques.
Source:
Recombinant corresponding to human NGF-beta expressed in E.coli.
Molecular weight:
~14kD
Biological Activity:
The ED50 as determined by the ability to stimulate neurite growth in chick E9 DRG cells is ~1.0 ng/ml.
Endotoxin:
~0.1ng/ug
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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